International Journal of Life Science and Engineering
Articles Information
International Journal of Life Science and Engineering, Vol.3, No.2, Jun. 2018, Pub. Date: Jun. 6, 2018
Isolation of Antibiotic Resistant Bacteria from Makelele River (Kinshasa, DR Congo) and Their Susceptibility Towards Plant-Derived Silver Nanoparticles
Pages: 25-38 Views: 734 Downloads: 218
Authors
[01] Koto-te-Nyiwa Ngbolua, Department of Biology, University of Kinshasa, Kinshasa City, Democratic Republic of the Congo; Department of Environmental Sciences, University of Gbadolite, Nord-Ubangi City, Democratic Republic of the Congo; Higher Pedagogical Institute of Abumombazi, Nord Ubangi City, Democratic Republic of the Congo.
[02] Gédéon Bongo, Department of Biology, University of Kinshasa, Kinshasa City, Democratic Republic of the Congo.
[03] Beaudrique Nsimba, Department of Chemistry, University of Kinshasa, Kinshasa City, Democratic Republic of the Congo.
[04] Emmanuel Lengbiye, Department of Biology, University of Kinshasa, Kinshasa City, Democratic Republic of the Congo.
[05] Jeff Iteku, Department of Biology, University of Kinshasa, Kinshasa City, Democratic Republic of the Congo.
[06] Pitchouna Kilunga, Department of Chemistry, University of Kinshasa, Kinshasa City, Democratic Republic of the Congo.
[07] Goslin Gafuene, Department of Biology, University of Kinshasa, Kinshasa City, Democratic Republic of the Congo.
[08] Colette Masengo Ashande, Department of Environmental Sciences, University of Gbadolite, Nord-Ubangi City, Democratic Republic of the Congo; Higher Pedagogical Institute of Abumombazi, Nord Ubangi City, Democratic Republic of the Congo.
[09] Tshiama Claudine, Department of Teaching and Administration in Nursing Care, Higher Institute of Medical Techniques, Kinshasa City, Democratic Republic of the Congo.
[10] Kongo Nzapo, Nursing Sciences, Red Cross Health Sciences Higher Institute, Kinshasa City, Democratic Republic of the Congo.
[11] Clément Inkoto, Department of Biology, University of Kinshasa, Kinshasa City, Democratic Republic of the Congo.
[12] Crispin Mulaji, Department of Chemistry, University of Kinshasa, Kinshasa City, Democratic Republic of the Congo.
[13] Nadège Ngombe, Pharmaceutical Sciences Faculty, University of Kinshasa, Kinshasa City, Democratic Republic of the Congo.
[14] Théophile Mbemba, Department of Biology, University of Kinshasa, Kinshasa City, Democratic Republic of the Congo.
[15] Pius Mpiana, Department of Chemistry, University of Kinshasa, Kinshasa City, Democratic Republic of the Congo.
Abstract
In Kinshasa city, Democratic Republic of the Congo (DRC) Rivers are highly affected by pollution mainly due to the discharge of garbage, domestic and industrial wastes without any prior treatment. The problem of waste management is a serious issue in this city. The main objective of the current research was to assess the antimicrobial activity of silver nanoparticles vis-à-vis certain bacteria indicative of faecal pollution from Makelele River. The preliminary characterization of silver nanoparticles was carried out using UV-visible spectrophotometer. Noble metals, such as silver nanoparticles, exhibit unique and adjustable optical properties due to their external plasmon resonance and the reduction of silver ions was monitored. The antibiotic susceptibility test results confirmed the inactivity of these antibiotics tested against the wild strain of E. coli and Enterococcus sp. The synthesized silver nanoparticles displayed a good antibacterial activity against Enterococcus sp. This synthesis is designed to bypass the situation of drug resistance and these results provide strong evidence that silver nanoparticles can be used to fight against antibiotic-resistant bacteria.
Keywords
Antibiotic Resistant Bacteria, Green Chemistry, Surface Water, Metallic Trace Elements, Pollution
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