International Journal of Life Science and Engineering
Articles Information
International Journal of Life Science and Engineering, Vol.1, No.4, Sep. 2015, Pub. Date: Aug. 3, 2015
Cytotoxic, Anthelmintic and Analgesic Activities of Methanol Extracts from Different Plant Parts of Tabernaemontana Corymbosa (Family: Apocynaceae)
Pages: 202-206 Views: 2447 Downloads: 1137
Authors
[01] Sumit Kumar Das, Department of Pharmacy, Stamford University Bangladesh, Dhaka, Bangladesh.
[02] Sadia Afreen Chowdhury, Department of Pharmacy, Stamford University Bangladesh, Dhaka, Bangladesh.
Abstract
The aim of the present study was to evaluate the cytotoxicity, anthelmintic activity, and analgesic properties of the leaf, flower and steam of Tabernaemontana corymbosa (Family: Apocynaceae). The cytotoxic activity of the plant extracts were performed by brine shrimp lethality bioassay in which, the methanol extracts of the leaf, flower and steam got LC50 value of 0.571, 0.350 and 0.517g/ml respectively, while LC50 value of vincristin sulphate was 0.35g/ml. Anthelmintic activity was performed by observing the time of paralysis and the time of death of earthworms Pheretima posthuma compared with the standard drug, albendazole. The range of minimum to maximum time of paralysis and death of albendazole at the conc. 100g/ml was 2min. 6sec. to 9min. 23sec. whereas, the range of minimum to maximum time of paralysis and death of the fresh leaf juice was 2min. 52sec. to 6min. 32sec., respectively. The results confirmed the presence of significant anthelmintic activity. Analgesic potential of the extracts were evaluated using tail-flick and hot plate tests to understand the central analgesic activity in mice (Swiss Albino mice) at 200 and 400mg/kg body weight doses. The results of the present study suggested that the extracts possess significant analgesic activity in mice, which is comparable to the standard drug, diclofenac-Na.The aim of the present study was to evaluate the cytotoxicity, anthelmintic activity, and analgesic properties of the leaf, flower and steam of Tabernaemontana corymbosa (Family: Apocynaceae). The cytotoxic activity of the plant extracts were performed by brine shrimp lethality bioassay in which, the methanol extracts of the leaf, flower and steam got LC50 value of 0.571, 0.350 and 0.517g/ml respectively, while LC50 value of vincristin sulphate was 0.35g/ml. Anthelmintic activity was performed by observing the time of paralysis and the time of death of earthworms Pheretima posthuma compared with the standard drug, albendazole. The range of minimum to maximum time of paralysis and death of albendazole at the conc. 100g/ml was 2min. 6sec. to 9min. 23sec. whereas, the range of minimum to maximum time of paralysis and death of the fresh leaf juice was 2min. 52sec. to 6min. 32sec., respectively. The results confirmed the presence of significant anthelmintic activity. Analgesic potential of the extracts were evaluated using tail-flick and hot plate tests to understand the central analgesic activity in mice (Swiss Albino mice) at 200 and 400mg/kg body weight doses. The results of the present study suggested that the extracts possess significant analgesic activity in mice, which is comparable to the standard drug, diclofenac-Na.
Keywords
Tabernaemontana Corymbosa, Brine Shrimp Lethality Bioassay, Anthelmintic Activity, Analgesic Activity, Vincristine Sulphate, Albendazole, Diclofenac-Na
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