Articles Information
                    
                    International Journal of Chemical and Biomolecular Science, Vol.1, No.2, Aug. 2015, Pub. Date: Jul. 10, 2015
                    Effect of Temperature and Storage on Vitamin C Content in Fruits Juice
                    
                     
                        
                    
                    
                    Pages: 17-21 Views: 9182 Downloads: 26957
                    Authors
                    
                    
                    
                        
                            
                                
                                
                                    [01] 
                                
                            
                            
                                Abubakar El-Ishaq, School of Science and Technology, Science Laboratory Technology Department, Chemistry/Biochemistry Unit, Federal Polytechnic, Damaturu Nigeria. 
                            
                            
                        
                            
                                
                                
                                    [02] 
                                
                            
                            
                                Simon Obirinakem, School of Science and Technology, Science Laboratory Technology Department, Chemistry/Biochemistry Unit, Federal Polytechnic, Damaturu Nigeria. 
                            
                            
                        
                     
                    Abstract
                    
                    
                    
                        The amount of Vitamin C content of some fresh fruit degradaded with time was investigated; Pineapple, Orange, Watermelon and Tomato stored under difference conditions. The juices from the samples were extracted and vitamin C content in the juice was analysed at room temperature, 400C and at seven (7) days storage by indophenols titration method. The result revealed that vitamin C content is more in Pineapple juice compared to Orange, Watermelon and Tomato juice as shown in Table 1 in this order; 49.38 ±1.875, 39.75 ±1.000, 27.50±1.250 and 19.13 ±1.630 mg/100ml respectively. The fresh juice were boiled at 400C and vitamin C content was determined. Result shows that; there is decreased in Vitamin C content after boiling as shown in Table 1; 26.25 ±1.750, 23.00 ±1.520, 16.70±1.420 and 10.83 ±2.600 mg/100ml respectively. The fresh juices were also stored at room temperature for 7 days and vitamin C content was determined. Result revealed that; there is loss of vitamin C content in fresh juice but not as much as the boiled juice Table1; 42.13 ±0.875, 31.50 ± 1.000, 21.63 ±1.625 and 15.63±0.625 mg/100ml respectively. The rate at which vitamin C is loss during storage depend on the type of storage methods employed.
                    
                    Keywords
                    
                    
                    
                        Scurvy, Antioxidant, Indophenol Titration, Vitamin C in Fresh Fruits, Vegetables, Stability and Damaturu
                    
                    References
                    
                    
                    
                        
                            
                                
                                
                                    [01]
                                
                            
                            
                                Abubakar, El-Ishaq and Bukar, A. Abubakar. (2012). Biochemistry for Tertiary Institutions. Management Network Resources.Damaturu.p123. 
                            
                            
                        
                            
                                
                                
                                    [02]
                                
                            
                            
                                Babalola, O.O, Tugbobo, O.S, and Daramola, A.S. (2010). Effect of Processing on the Vitamin C Content of Seven Nigerian Green Leafy Vegetables. Advance Journal of Food Science and Technology. 2(6). pp303-305. 
                            
                            
                        
                            
                                
                                
                                    [03]
                                
                            
                            
                                AOAC. (2006).Official Method 967.21e Ascorbic Acid in Vitamin Preparations and Juices: 2,6-Dichloroindophenol Titrimetric Method.Official Methods of Analysis of the Association of Official Analytical Chemists. 
                            
                            
                        
                            
                                
                                
                                    [04]
                                
                            
                            
                                Berg, JM, Tymoczko, JL, Stryer L. Biochemistry. 5th edition. New York: W H Freeman; (2002). Available from http://www.ncbi.nlm.nih.gov/books/NBK21154/ 
                            
                            
                        
                            
                                
                                
                                    [05]
                                
                            
                            
                                Bethlehem Brown Campbell and Farrel Torrell. (2013).Introduction to Organic and Biochemistry.8th ed. Brooks/Cole. CENGAGE Learning. Belmont. USA. 
                            
                            
                        
                            
                                
                                
                                    [06]
                                
                            
                            
                                Davey, MW, Van Monatgu M, Sanmatin M, Kanellis A, Smirnoff N, Benzie IJJ, Strain JJ, Favell D, Fletcher J. (2000). Plant L-ascorbic acid: Chemistry, Function, Metabolism, Bioavailability and Effects of Processing. J Sci Food Agric.; 80: 825–860. 
                            
                            
                        
                            
                                
                                
                                    [07]
                                
                            
                            
                                Duyff, F. Ruurd, Joan Van Bosch, D Martin Laman, Bert-Jan Potter Van Loon, Wim H.J.P. Linssen. (2000) Neuromuscular Findings in Thyroid Dysfunction: A Prospective Clinical and Electrodiagnostic Study. J Neurol Neurosurgery Psychiatry; 68750-755 doi:10.1136/jnnp.68.6.750 
                            
                            
                        
                            
                                
                                
                                    [08]
                                
                            
                            
                                Frank, A.A., Cluster, T.J., Arakaki, C., and Murphy, S.P. (2004). Vitamin C and Flavonoids Level of Fruits and Vegetables Consumed in Hawaii. Journal of Food Composition and Analysis, 17, 1-35. 
                            
                            
                        
                            
                                
                                
                                    [09]
                                
                            
                            
                                Grano, A. De Tullio M.C. (2007). Ascorbic Acid as a Sensor of Oxidative Stress and a Regulator of Gene Expression: The Yin and Yang of vitamin C. Med Hypoth 69: 953-954. 
                            
                            
                        
                            
                                
                                    [10]
                                
                                
                            
                            
                                Johnson, Ojo Rotimi; Yetu, Abaya Jemimah; Oloruntoba, Akintayo Christopher; Samuel, Seriki. (2013). Effects of Nigerian Market Storage Conditions on Ascorbic Acid Contents of Selected Tetrapak Packaged Citrus Fruit Juice. Journal of Agricultural & Biological Science ; Feb 2013, Vol. 8 Issue 2, p179. 
                            
                            
                        
                            
                                
                                    [11]
                                
                                
                            
                            
                                Kiple, F. Kenneth. (2000). Cambridge World History of Food. 2 Volume 2. Cambridge University Press. page-1120. 
                            
                            
                        
                            
                                
                                    [12]
                                
                                
                            
                            
                                Padayatty, S.J., Katz, A, Wang Y et al., (2003). Vitamin C. Journal of American Coll.Nutr.2003.22; pp18-35. 
                            
                            
                        
                            
                                
                                    [13]
                                
                                
                            
                            
                                Maria, I.Gil, Encarna Aguayo, and Adel A. Kader. (2006). Quality Changes and Nutrient Retention in Fresh-Cut versus Whole Fruits during Storage. J. Agric. Food Chem. Vol 54 (12).pp4284-4296. 
                            
                            
                        
                            
                                
                                    [14]
                                
                                
                            
                            
                                Murcia, M. A., Lopez-Ayerra, B., Martinez-Tome, M., Vera, A. M., & Garcia-Carmona, F. (2000). Evolution of Ascorbic Acid and Peroxidase During Industrial Processing of Broccoli. Journal of the Science of Food and Agriculture, 80, 1882–1886. 
                            
                            
                        
                            
                                
                                    [15]
                                
                                
                            
                            
                                Murray, R. K., Bender D. A., Botham K. M., Kennelly P. J., Rodwell V. W., Weil P. A. (2012). A Lange Medical Book: Harper's Illustrated Biochemistry. New York, NY: Mc Graw Hill Medical 
                            
                            
                        
                            
                                
                                    [16]
                                
                                
                            
                            
                                Rickman, J.C, Bruhn, C.M, and Barrett DM. (2007). Nutritional Comparison of Fresh, Frozen, and Canned fruits and Vegetables II. Vitamin A and Carotenoids, Vitamin E, Minerals and Fiber. Journal of the Science of Food and Agriculture; 87: 1185-1196. 
                            
                            
                        
                            
                                
                                    [17]
                                
                                
                            
                            
                                Schorah, CJ,C. Downing, A. Piritsi et al., (1996). Total Vitamin C, Ascorbic Acid, and Hydro Ascorbic Acid Concentration in Plasma of Critical Ill Patients, Am J Nutr 63:760-765