[01] Serkan Dayan, Erciyes University, Faculty of Science, Department of Chemistry, Kayseri, Turkey. [02] Burcu Somturk, Erciyes University, Faculty of Science, Department of Chemistry, Kayseri, Turkey. [03] Nilgun Kayaci, Erciyes University, Faculty of Science, Department of Chemistry, Kayseri, Turkey. [04] Nalan Ozdemir, Erciyes University, Faculty of Science, Department of Chemistry, Kayseri, Turkey. [05] Nilgun Kalaycioglu Ozpozan, Erciyes University, Faculty of Science, Department of Chemistry, Kayseri, Turkey. [06] Hasan Ozdemir, Ataturk University, Faculty of Science, Department of Chemistry, Erzurum, Turkey.

A new series of [N-(3-aminophenyl)-arylsulfonamides] hydrochloride (Aryl= 4-methoxybenzene, 4-tert-butylbenzene, 4-nitrobenzene, 4-chlorobenzene) compounds (1-4) were synthesized successfully with a simple method. The compounds (1-4) were elucidated on the basis of elemental and spectral analyses (NMR, FT-IR, EA) and their inhibitory effects on the activity of purified peroxidase (POD) enzyme were evaluated by UV-vis spectrophotometers. The POD enzyme was purified from red cabbage (Brassica oleracea var. capitata f. rubra) using affinity chromatography. The half maximal inhibitory concentration (IC50) values were found to be 0.144 mM, 0.2 mM, 0.117 mM and 0.185 mM for compounds 1, 2, 3 and 4 respectively. The inhibition results show that all the compounds (1-4) inhibited POD enzyme activity. Particular, the N-(3-aminophenyl)-4-nitrobenzenesulfonamide hydrochloride compound (3) was found as the most active compound for POD compared to the others.

Sulfonamide, Peroxidase, Inhibition, Synthesis

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