Agricultural and Biological Sciences Journal
Articles Information
Agricultural and Biological Sciences Journal, Vol.6, No.1, Mar. 2020, Pub. Date: Jan. 14, 2020
RT-qPCR Expression of Fatty Acid Binding Protein1 and 2 (FABP1&2) Genes in Fatty Liver Tissue Overfed Goose
Pages: 22-26 Views: 1164 Downloads: 305
Authors
[01] Rashid Habiballa Osman, Faculty of Animal Production, West Kordofan University, El-Nuhud, Sudan; Faculty of Animal Science and Technology, Yangzhou University, Yangzhou, China.
[02] Mojahid Abdallah Abdalhag, Faculty of Animal Science and Technology, Yangzhou University, Yangzhou, China; Faculty of Agricultural Technology & Fish Science, Al Neelain University, Khartoum, Sudan.
[03] Tamador Algam, Faculty of Agricultural Technology & Fish Science, Al Neelain University, Khartoum, Sudan.
[04] Ahmed Kamel, Faculty of Veterinary Science, Seuz Canal University, Seuz, Egypt.
[05] Wafaa Babiker Zomrawi, Faculty of Agriculture and Natural Resources, University of Bakht Elruda, Al-Dueim, Sudan.
[06] Mohammed Ibrahim Ahmed, Faculty of Veterinary Science, West Kordofan University, El-Nuhud, Sudan.
[07] Dao Qing Gong, Faculty of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Abstract
Fatty acid-binding proteins 1, 2, 3, 4 and L-FABP occur in the liver in a high concentration where it is involved in the fatty liver functions. In this study, diet content cooked maize was used to overfeed Landes geese for 19 days. The expression levels of FABP1/2 were determined by using quantitative PCR in goose liver and primary hepatocytes. The expression level of FABP2 gradually decreased with overfeeding time in the livers of the geese, while FABP1 was extremely down-regulated after one week of overfed. The expression level of FABPs1 mRNA was the lowest in the overfeeding group at 89 days. In primary hepatocytes, the data indicated that the expression of FABP1/2 were inhibited by high levels of glucose (25-50 mM). In addition, insulin (50-100mM) inhibited FABP1 in goose hepatocytes, while it was not significantly (p 0.05) for FABP2. These findings suggest that the expression of the FABP1 gene is hyper-effect to hyperglycemia and hyperinsulinemia that causing inhibition of the gene in goose fatty liver. For fatty acid treatment, the expression levels of FABP1/2 were not significantly altered in fatty acid (0.25 or 0.5mM) treatment. All data were normalized by the GAPDH gene and analyzed by the 2-ΔΔCt method.
Keywords
Fatty Liver, Goose, FABP 1/2 genes, Overfeeding, Primary Hepatocytes
References
[01] R. K. Ockner, J. A. Manning, R. B. Poppenhausen, W. K. Ho, A binding protein for fatty acids in cytosol of intestinal mucosa, liver, myocardium, and other tissues, Science 177 (1972) 56-58.
[02] G. V. Richieri, R. T. Ogata, A. M. Kleinfeld, Equilibrium constants for the binding of fatty acids with fatty acid-binding proteins from adipocyte, intestine, heart, and liver measured with the fluorescent probe ADIFAB, Journal of Biological Chemistry 269 (1994) 23918-23930.
[03] M. Furuhashi, G. S. Hotamisligil, Fatty acid-binding proteins: role in metabolic diseases and potential as drug targets, Nature reviews Drug discovery 7 (2008) 489-503.
[04] G. S. Hotamisligil, E. Erbay, Nutrient sensing and inflammation in metabolic diseases, Nature Reviews Immunology 8 (2008) 923-934.
[05] J. Storch, A. E. Thumser, Tissue-specific functions in the fatty acid-binding protein family, Journal of Biological Chemistry 285 (2010) 32679-32683.
[06] L. Lawrie, S. Dundas, S. Curran, G. Murray, Liver fatty acid binding protein expression in colorectal neoplasia, British journal of cancer 90 (2004) 1955-1960.
[07] J. Pang, W.-P. Liu, X.-P. Liu, L.-Y. Li, Y.-Q. Fang, Q.-P. Sun, S.-J. Liu, M.-T. Li, Z.-L. Su, X. Gao, Profiling protein markers associated with lymph node metastasis in prostate cancer by DIGE-based proteomics analysis, Journal of proteome research 9 (2009) 216-226.
[08] B. P. Atshaves, G. G. Martin, H. A. Hostetler, A. L. McIntosh, A. B. Kier, F. Schroeder, Liver fatty acid-binding protein and obesity, The Journal of nutritional biochemistry 21 (2010) 1015-1032.
[09] C. Shu, B. Wang, Z. Li, W. Ge, M. Zhang, B. Yue, Fatty acids deposition and FAS mRNA expression abundance in liver tissue of overfeeding goose, China Agric. Sci 45 (2012) 2002-2011.
[10] C. Han, J. Wang, L. Li, Z. Zhang, L. Wang, Z. Pan, The role of insulin and glucose in goose primary hepatocyte triglyceride accumulation, Journal of Experimental Biology 212 (2009) 1553-1558.
[11] R. H. Osman, L. Liu, L. Xia, X. Zhao, Q. Wang, X. Sun, Y. Zhang, B. Yang, Y. Zheng, D. Gong, Fads 1 and 2 are promoted to meet instant need for long-chain polyunsaturated fatty acids in goose fatty liver, Molecular and cellular biochemistry 418 (2016) 103-117.
[12] R. Zhang, L. Zhu, Y. Zhang, D. Shao, L. Wang, D. Gong, cDNA cloning and the response to overfeeding in the expression of stearoyl-CoA desaturase 1 gene in Landes goose, Gene 512 (2013) 464-469.
[13] K. J. Livak, T. D. Schmittgen, Analysis of relative gene expression data using real-time quantitative PCR and the 2 (-Delta Delta C (T)) Method, Methods 25 (2001) 402-408.
[14] R. L. Smathers, D. R. Petersen, The human fatty acid-binding protein family: evolutionary divergences and functions, Human genomics 5 (2011) 1.
[15] J. F. Glatz, J. H. Veerkamp, Intracellular fatty acid-binding proteins, International Journal of Biochemistry 17 (1985) 13-22.
[16] S. Fujii, H. Kawaguchi, H. Yasuda, Fatty acid binding protein in kidney of normotensive and genetically hypertensive rats, Hypertension 10 (1987) 93-99.
[17] G. Wang, Y. Gong, J. Anderson, D. Sun, G. Minuk, M. S. Roberts, F. J. Burczynski, Antioxidative function of L‐FABP in L‐FABP stably transfected Chang liver cells, Hepatology 42 (2005) 871-879.
[18] R. R. Brenner, Hormonal modulation of Δ6 and Δ5 desaturases: case of diabetes, Prostaglandins, leukotrienes and essential fatty acids 68 (2003) 151-162.
[19] H. P. Cho, M. T. Nakamura, S. D. Clarke, Cloning, expression, and nutritional regulation of the mammalian Δ-6 desaturase, Journal of Biological Chemistry 274 (1999) 471-477.
[20] H. P. Cho, M. Nakamura, S. D. Clarke, Cloning, expression, and fatty acid regulation of the human Δ-5 desaturase, Journal of Biological Chemistry 274 (1999) 37335-37339.
[21] V. Wijendran, I. Downs, C. T. Srigley, K. S. Kothapalli, W. J. Park, B. S. Blank, J. P. Zimmer, C. Butt, N. Salem, J. T. Brenna, Dietary arachidonic acid and docosahexaenoic acid regulate liver fatty acid desaturase (FADS) alternative transcript expression in suckling piglets, Prostaglandins, Leukotrienes and Essential Fatty Acids (PLEFA) 89 (2013) 345-350.
[22] T. Melin, Å. Nilsson, Delta-6-desaturase and delta-5-desaturase in human Hep G2 cells are both fatty acid interconversion rate limiting and are upregulated under essential fatty acid deficient conditions, Prostaglandins, leukotrienes and essential fatty acids 56 (1997) 437-442.
[23] S. Dharmarajan, E. P. Newberry, G. Montenegro, I. Nalbantoglu, V. R. Davis, M. J. Clanahan, V. Blanc, Y. Xie, J. Luo, J. W. Fleshman, Liver fatty acid-binding protein (L-Fabp) modifies intestinal fatty acid composition and adenoma formation in ApcMin/+ mice, Cancer Prevention Research 6 (2013) 1026-1037.
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